One step Urine Amphetamine test

Introduction

Amphetamine is a central nervous system stimulant. It is a controlled substance, as defined by the National Institute on Drug Abuse (NIDA).

Test Amphetamine is an immuno-chromatographic in vitro assay for presumptive qualitative determination of amphetamine in urine. The test is simple, with visual reading of results without additional instrumentation.

Test Amphetamine allows to detect amphetamine concentrations of 500 ng/ml and higher. The test kit allows performing 50 tests. It contains 50 sealed foil pouches, with one immunochromatographic testing device and one disposable sample dropper in each pouch. A testing device contains a test membrane with anti-amphetamine antibodies and amphetamine in a stabilizing matrix containing proteins and sodium azide, enclosed in plastic casing.

The result is registered by observing the test ("T") line and control ("C") line in the window. Distinctive control line also serves as an indicator of proper performance of the testing device.

Test Amphetamine is for in vitro diagnostic use only.

STORAGE CONDITIONS

Store below 28°C; do not freeze.

SAMPLE COLLECTION

Collect a urine sample in a clean, dry container. For best results, perform the test on the same day. If necessary, the sample may be stored in a refrigerator for up to 48 hours, or frozen for prolonged storage.

Note: Do not open the foil pouch until you are ready to perform the test. If a sample or a test kit was refrigerated, bring unopened pouch and test sample to room temperature before performing the test.

TEST PROCEDURE AND INTERPRETATION OF RESULTS

Test Procedure

1. Open a foil pouch by tearing along the "notch". Remove the testing device and the sample dropper. Place the testing device on a flat horizontal surface.

2. Holding the dropper vertically, carefully dispense exactly four drops of urine into the sample well (marked "S") of the testing device.

3. Read the test results at five minutes.

Interpretation of the test results

Observe the two color lines (bands): the control line in the control ("C") region of the test membrane, and the test line in the test ("T") region of the membrane.

POSITIVE

The control line appears in the test window, but the test line is not visible. Amphetamine concentration in the test sample is 500 ng/ml or more.

NEGATIVE

Two pink-rose lines (bands) are visible in the control ("C") and test ("T") areas of the test window. Amphetamine level in the urine sample is below the Test Amphetamine sensitivity of 500 ng/ml.

INVALID

The test is invalid if the control line is not visible at five minutes. The test failed, or the test procedure was not followed properly. Verify the test procedure and repeat the test with a new testing device.

Note: Do not interpret Test Amphetamine results after more than five minutes. To avoid confusion, discard the testing device after registering the test result at five minutes.